Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Name: Anti-STAT5 (phospho Tyr694) Antibody [ARC0176]
Brand: Antibodies.com
SKU: A305577

Enlarge Image

This product is discontinued

Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) has been discontinued and is no longer available.

View all Anti-STAT5 Antibodies.

Specifications

Name

Anti-STAT5 (phospho Tyr694) Antibody [ARC0176]

Description

Rabbit monoclonal [ARC0176] antibody to STAT5 (phospho Tyr694).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around Y694 of human Stat5 (P42229).

Sequence

DGYVK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0176

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

MGF, Signal transducer and activator of transcription 5A, Signal Transducer and Activator of Transcription 5B, STA5A_HUMAN, STAT 5A, STAT 5B, STAT5A, STAT5B, Transcription factor STAT5A, Transcription factor STAT5B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Western blot analysis of TF-1 cells, using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at 1:1,000 dilution. TF-1 cells were treated by GM-CSF (25 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Publishing research using Anti-STAT5 (phospho Tyr694) Antibody [ARC0176] (A305577)? Please let us know so that we can list the citation on this page.