Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524)

Name: Anti-Stromal interaction molecule 1 Antibody [ARC57102]
Brand: Antibodies.com
SKU: A308524
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC57102] antibody to Stromal interaction molecule 1 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Stromal interaction molecule 1 Antibody [ARC57102]

Description

Rabbit monoclonal [ARC57102] antibody to Stromal interaction molecule 1.

Applications

WB, IHC, IP

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 530-685 of human STIM1 (NP_003147.2).

Sequence

RQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC57102

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

D11S4896E, GOK, OTTHUMP00000164512, OTTHUMP00000229140, OTTHUMP00000230742, SIM, STIM 1, STIM1, Stim1 stromal interaction molecule 1, STIM1L, STIM1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524)

Western blot analysis of various lysates, using Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524). This Western blot was performed on the immunoprecipitate using Anti-Stromal interaction molecule 1 Antibody [ARC57102] (A308524) at a dilution of 1:1000.

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