Sumo1 Cell Based ELISA Kit (A103122)

Sumo1 Cell Based ELISA Kit

Cell-based (qualitative)

This Sumo1 Cell Based ELISA Kit allows for the detection of Sumo1 and the effects that certain stimulation conditions have on Sumo1 expression in different cell lines. Qualitative determination of Sumo1 concentration is achieved by an indirect ELISA format. In essence, the Sumo1 is captured by Anti-Sumo1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Sumo1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Colorimetric

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

DAP1, GAP modifying protein 1, GAP-modifying protein 1, GMP 1, GMP1, OFC10, PIC 1, PIC1, SENP2, Sentrin, Sentrin 1, Small ubiquitin related modifier 1, Small ubiquitin-like modifier 1, Small ubiquitin-related modifier 1, SMT3, SMT3 homolog 3, SMT3 suppressor of mif two 3 homolog 1, SMT3, yeast, homolog 3, Smt3C, SMT3H3, Sumo 1, SUMO-1, SUMO1_HUMAN, Ubiquitin homology domain protein PIC1, Ubiquitin Like 1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Ubiquitin-like protein UBL1, UBL 1, UBL1