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Anti-Superoxide Dismutase 1 Antibody (A12593)

Western Blot - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.com Immunohistochemistry - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.com Immunohistochemistry - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.com Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Superoxide Dismutase 1 for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Superoxide Dismutase 1 Antibody
Description
Rabbit polyclonal antibody to Superoxide Dismutase 1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2-154 of human SOD1 (NP_000445.1).
Sequence
ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
16 - 18 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ALS, ALS1, Amyotrophic Lateral Sclerosis 1 (Adult), Amyotrophic lateral sclerosis 1 adult, Cu,Zn Superoxide Dismutase, EC 1.15.1.111, Cu/Zn SOD, Cu/Zn superoxide dismutase, Epididymis secretory protein Li 44, HEL S 44, Homodimer, hSod1, Indophenoloxidase A, IPOA, Mn superoxide dismutase, SOD, SOD soluble, SOD1, SOD2, SODC, SODC_HUMAN, Superoxide dismutase 1 soluble, Superoxide Dismutase 1, Soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic, Superoxide dismutase [Cu-Zn], Superoxide Dismutase, Cystolic
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Superoxide Dismutase 1 Antibody (A12593)
Western blot analysis of extracts of various cell lines, using Anti-Superoxide Dismutase 1 Antibody (A12593) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Superoxide Dismutase 1 Antibody (A12593)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Superoxide Dismutase 1 Antibody (A12593) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Superoxide Dismutase 1 Antibody (A12593)
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Superoxide Dismutase 1 Antibody (A12593) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A12593) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A12593)
Immunofluorescence analysis of NIH/3T3 cells using Anti-Superoxide Dismutase 1 Antibody (A12593) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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