Anti-SUR1 Antibody [S289-16] (A304817)

Name: Anti-SUR1 Antibody [S289-16]
Brand: Antibodies.com
SKU: A304817
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S289-16] antibody to SUR1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-SUR1 Antibody [S289-16]

Description

Mouse monoclonal [S289-16] antibody to SUR1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Hamster

Cross Reactivity

This antibody does not cross-react with SUR2B.

Immunogen

Fusion protein amino acids 1548-1582 (cytoplasmic C-terminus) of rat SUR1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S289-16

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~160 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ABC36, Abcc8, ABCC8_HUMAN, ATP binding cassette sub family C (CFTR/MRP) member 8, ATP binding cassette transporter sub family C member 8 (1), ATP-binding cassette sub-family C member 8, HHF1, HI, HRINS, MRP8, PHHI, Sulfonylurea receptor (hyperinsulinemia), Sulfonylurea receptor 1, SUR, SUR1delta2, TNDM2

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-SUR1 Antibody [S289-16] (A304817)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-SUR1 Antibody [S289-16] (A304817), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-SUR1 Antibody [S289-16] (A304817)

Western blot analysis of rat brain membrane showing detection of ~160 kDa SUR1 protein using Anti-SUR1 Antibody [S289-16] (A304817) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~160 kDa.

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Figure 3: Immunofluorescence - Anti-SUR1 Antibody [S289-16] (A304817)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-SUR1 Antibody [S289-16] (A304817), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SUR1 Antibody. (D) Composite.

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