Anti-Syndecan-1 Antibody [ARC0918] (A308330)

Name: Anti-Syndecan-1 Antibody [ARC0918]
Brand: Antibodies.com
SKU: A308330
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0918] antibody to Syndecan-1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Syndecan-1 Antibody [ARC0918]

Description

Rabbit monoclonal [ARC0918] antibody to Syndecan-1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 23-254 of human Syndecan 1 (P18827).

Sequence

QIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0918

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

90 - 100 kDa / 180 - 230 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD138, CD138 antigen, CD_antigen, heparan sulfate proteoglycan fibroblast growth factor receptor, SDC, Sdc1, SDC1_HUMAN, SYND1, Syndecan, Syndecan 1, syndecan proteoglycan 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Syndecan-1 Antibody [ARC0918] (A308330)

Western blot analysis of extracts of various cell lines, using Anti-Syndecan-1 Antibody [ARC0918] (A308330) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Syndecan-1 Antibody [ARC0918] (A308330)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Syndecan-1 Antibody [ARC0918] (A308330) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Syndecan-1 Antibody [ARC0918] (A308330)

Immunofluorescence analysis of A431 cells using Anti-Syndecan-1 Antibody [ARC0918] (A308330) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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