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Anti-Syntaxin 4 Antibody [ARC2113] (A308390)

Western Blot - Anti-Syntaxin 4 Antibody [ARC2113] (A308390) - Antibodies.com Western Blot - Anti-Syntaxin 4 Antibody [ARC2113] (A308390) - Antibodies.com Immunofluorescence - Anti-Syntaxin 4 Antibody [ARC2113] (A308390) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2113] antibody to Syntaxin 4 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Syntaxin 4 Antibody [ARC2113]
Description
Rabbit monoclonal [ARC2113] antibody to Syntaxin 4.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Syntaxin 4 (Q12846).
Sequence
QTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYRE
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2113
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
NY REN 31 antigen, p35 2, PLACENTAL, Renal carcinoma antigen NY REN 31, Renal carcinoma antigen NY-REN-31, Stx4, STX4A, STX4_HUMAN, Syntaxin 4A, syntaxin 4A (placental), Syntaxin-4
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Syntaxin 4 Antibody [ARC2113] (A308390) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Syntaxin 4 Antibody [ARC2113] (A308390)
Western blot analysis of extracts of various cell lines, using Anti-Syntaxin 4 Antibody [ARC2113] (A308390) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Syntaxin 4 Antibody [ARC2113] (A308390) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Syntaxin 4 Antibody [ARC2113] (A308390)
Western blot analysis of extracts of Rat brain, using Anti-Syntaxin 4 Antibody [ARC2113] (A308390) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-Syntaxin 4 Antibody [ARC2113] (A308390) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Syntaxin 4 Antibody [ARC2113] (A308390)
Immunofluorescence analysis of NIH-3T3 cells using Anti-Syntaxin 4 Antibody [ARC2113] (A308390) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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