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Synthetic Nanodisc Human CD151 Protein (A318436)

ELISA - Synthetic Nanodisc Human CD151 Protein (A318436) - Antibodies.com SDS-PAGE - Synthetic Nanodisc Human CD151 Protein (A318436) - Antibodies.com Synthetic Nanodisc Human CD151 Protein (A318436) - Antibodies.com
$1,300
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7-8 Business Days

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Name
Synthetic Nanodisc Human CD151 Protein
Description
Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human CD151 protein to be purified and analysed in vitro.
Applications
ELISA, SDS-PAGE
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CD151 protein has a MW of 28.3 kDa.
Conjugate

Unconjugated

Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyms
CD151 antigen, CD151 molecule, CD151 molecule (Raph blood group), CD151_HUMAN, GP27, hemidesmosomal tetraspanin CD151, Membrane glycoprotein SFA-1, MER2, PETA-3, PETA3, Platelet endothelial tetraspan antigen 3, platelet surface glycoprotein gp27, Platelet-endothelial cell tetraspan antigen 3, Platelet-endothelial tetraspan antigen 3, RAPH, Red blood cell antigen MER2, SFA 1, SFA1, Tetraspanin-24, Tspan-24, TSPAN24
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
ELISA - Synthetic Nanodisc Human CD151 Protein (A318436) - Antibodies.comEnlarge Image
Figure 1: Synthetic Nanodisc Human CD151 Protein (A318436)
ELISA plates were pre-coated with Synthetic Nanodisc Human CD151 Protein (A318436) (0.2 µg/well). Serial diluted Anti-Flag Tag Antibody solutions were added, washed, and incubated with secondary antibody before ELISA reading. From this data, the EC50 for Anti-Flag Tag Antibody binding with CD151-Nanodisc is 4.446 µg/ml.
SDS-PAGE - Synthetic Nanodisc Human CD151 Protein (A318436) - Antibodies.comEnlarge Image
Figure 2: SDS-PAGE - Synthetic Nanodisc Human CD151 Protein (A318436)
Synthetic Nanodisc Human CD151 Protein (A318436) on SDS-PAGE under reducing conditions.
Synthetic Nanodisc Human CD151 Protein (A318436) - Antibodies.comEnlarge Image
Figure 3: Synthetic Nanodisc Human CD151 Protein (A318436)
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
Publishing research using Synthetic Nanodisc Human CD151 Protein (A318436)? Please let us know so that we can list the citation on this page.
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