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Anti-TAGLN / Transgelin Antibody (A9989)

Western Blot - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.com Western Blot - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.com Immunohistochemistry - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.com Immunohistochemistry - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.com
This product is discontinued

Anti-TAGLN / Transgelin Antibody (A9989) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-TAGLN / Transgelin Antibody
Description
Rabbit polyclonal antibody to TAGLN / Transgelin.
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-201 of human Transgelin (TAGLN) (NP_001001522.1).
Sequence
MANKGPSYGMSREVQSKIEKKYDEELEERLVEWIIVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPDGSKPVKVPENPPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLFEGKDMAAVQRTLMALGSLAVTKNDGHYRGDPNWFMKKAQEHKREFTESQLQEGKHVIGLQMGSNRGASQAGMTGYGRPRQIIS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
23 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.comEnlarge Image

Western Blot - Anti-TAGLN / Transgelin Antibody (A9989)

Western blot analysis of extracts of Rat uterus, using Anti-TAGLN / Transgelin Antibody (A9989) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.comEnlarge Image

Western Blot - Anti-TAGLN / Transgelin Antibody (A9989)

Western blot analysis of extracts of Mouse uterus, using Anti-TAGLN / Transgelin Antibody (A9989) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-TAGLN / Transgelin Antibody (A9989)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-TAGLN / Transgelin Antibody (A9989) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-TAGLN / Transgelin Antibody (A9989) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-TAGLN / Transgelin Antibody (A9989)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-TAGLN / Transgelin Antibody (A9989) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.