Anti-Tbp7 Antibody [ARC58702] (A309870)

Name: Anti-Tbp7 Antibody [ARC58702]
Brand: Antibodies.com
SKU: A309870

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This product is discontinued

Anti-Tbp7 Antibody [ARC58702] (A309870) has been discontinued and is no longer available.

View all Anti-Tbp7 Antibodies.

Specifications

Name

Anti-Tbp7 Antibody [ARC58702]

Description

Rabbit monoclonal [ARC58702] antibody to Tbp7.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:9,000, IHC: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human PSMC4.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58702

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

26S protease regulatory subunit 6B, 26S proteasome AAA ATPase subunit RPT3, 26S proteasome AAA-ATPase subunit RPT3, MB67 interacting protein, MB67-interacting protein, MIP224, Protease 26S subunit 6, Proteasome (prosome macropain) 26S subunit ATPase 4, Proteasome 19S S6, Proteasome 26S subunit ATPase 4, Proteasome 26S subunit, ATPase, 4, PRS6B_HUMAN, PSMC4, RPT3, S6, Tat binding protein 7, TAT-binding protein 7, TBP 7, TBP-7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Tbp7 Antibody [ARC58702] (A309870)

Western blot analysis of HeLa, using Anti-Tbp7 Antibody [ARC58702] (A309870) at 1:8,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Tbp7 Antibody [ARC58702] (A309870)

Western blot analysis of 293F, using Anti-Tbp7 Antibody [ARC58702] (A309870) at 1:8,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Western Blot - Anti-Tbp7 Antibody [ARC58702] (A309870)

Western blot analysis of various lysates, using Anti-Tbp7 Antibody [ARC58702] (A309870) at 1:8,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 4: Immunohistochemistry - Anti-Tbp7 Antibody [ARC58702] (A309870)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Tbp7 Antibody [ARC58702] (A309870) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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