Anti-TCPTP Antibody (A13672)

Name: Anti-TCPTP Antibody
Brand: Antibodies.com
SKU: A13672
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to TCPTP for WB and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-TCPTP Antibody

Description

Rabbit polyclonal antibody to TCPTP.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human PTPN2 (P17706).

Sequence

MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSESALRKRI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Protein tyrosine phosphatase non receptor type 2, PTN2, PTN2_HUMAN, PTPN 2, Ptpn2, PTPT, T cell protein tyrosine phosphatase, T-cell protein-tyrosine phosphatase, TC PTP, TCELLPTP, Tyrosine protein phosphatase non receptor type 2, Tyrosine-protein phosphatase non-receptor type 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-TCPTP Antibody (A13672)

Western blot analysis of extracts of HeLa cells, using Anti-TCPTP Antibody (A13672) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-TCPTP Antibody (A13672)

Western blot analysis of extracts from normal (control) and PTPN2 knockout (KO) A-549 cells, using Anti-TCPTP Antibody (A13672) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunofluorescence - Anti-TCPTP Antibody (A13672)

Immunofluorescence analysis of A-549 cells using Anti-TCPTP Antibody (A13672) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-TCPTP Antibody (A13672)

Immunofluorescence analysis of HeLa cells using Anti-TCPTP Antibody (A13672) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).