Anti-TCR alpha/beta Antibody [H57-597] (FITC) (A280564)

Anti-TCR alpha/beta Antibody [H57-597] (FITC)

Hamster monoclonal [H57-597] antibody to TCR alpha/beta (FITC).

This antibody recognises the beta-chain of the murine alpha/beta T cell receptor and does not show any reactivity to TCR gamma/delta (Gascoigne 1990).Clone H57-597 has been reported to activate T-cells in immobilized form and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.

Flow Cytometry: 1:5 - 1:10 Pack Size: 0.1 mgNeat Pack Size: 0.5 mg, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Mouse

Affinity purified murine T cell receptor.

Hamster

Monoclonal

IgG

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Hamster anti mouse TCR a/ß, clone H57-597, recognizes the ß-chain of the murine a/ß T cell receptor and does not show any reactivity to TCR?/d (Gascoigne 1990).Clone H57-597 has been reported to activate T-cells in immobilized form (Kubo et al. 1989) and has also been used for in vivo cell depletion (van der Heyde et al., 1995 and Skeen & Ziegler 1993). Removal of sodium azide is recommended prior to using this clone in functional assays.

This product is for research use only. It is not intended for diagnostic or therapeutic use.