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Anti-TCR beta Constant 1 Antibody [JOVI.1] (FITC) (A242893)

Flow Cytometry - Anti-TCR C beta 1 Antibody [JOVI.1] (FITC) (A242893) - Antibodies.com Flow Cytometry - Anti-TCR C beta 1 Antibody [JOVI.1] (FITC) (A242892) - Antibodies.com Flow Cytometry - Anti-TCR C beta 1 Antibody [JOVI.1] (FITC) (A242893) - Antibodies.com
$285
Product Datasheet

Mouse monoclonal [JOVI.1] antibody to TCR beta Constant 1 (FITC) for Flow Cytometry.

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Alternative Formats and ConjugatesAlternative Formats

Freight Charges
$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-TCR beta Constant 1 Antibody [JOVI.1] (FITC)
Description
Mouse monoclonal [JOVI.1] antibody to TCR beta Constant 1 (FITC).
Specificity
This antibody recognizes an extracellular epitope on TCR C beta 1.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR V beta 3 C beta 1.
Host
Mouse
Clonality
Monoclonal
Clone ID
JOVI.1
Isotype
IgG2a
Light Chains
kappa
Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Anti-TCR C beta 1 Antibody [JOVI.1] (FITC) (A242893) - Antibodies.comEnlarge Image
Figure 1: Flow Cytometry - Anti-TCR beta Constant 1 Antibody [JOVI.1] (FITC) (A242893)
Flow cytometry surface staining pattern of human peripheral whole blood stained using Anti-TCR C beta 1 Antibody (FITC) (4 µl reagent / 100 µl of peripheral whole blood).
Flow Cytometry - Anti-TCR C beta 1 Antibody [JOVI.1] (FITC) (A242892) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-TCR beta Constant 1 Antibody [JOVI.1] (FITC) (A242893)
Flow cytometry multicolor surface staining of human lymphocytes stained using Anti-TCR C beta 1 Antibody (FITC) (4 µl reagent / 100 µl of peripheral whole blood) and Anti-CD3 Antibody [UCHT1] (APC) (10 µl reagent / 100 µl of peripheral whole blood).
Flow Cytometry - Anti-TCR C beta 1 Antibody [JOVI.1] (FITC) (A242893) - Antibodies.comEnlarge Image
Figure 3: Flow Cytometry - Anti-TCR beta Constant 1 Antibody [JOVI.1] (FITC) (A242893)
Separation of human TCR Cbeta1 positive T cells (red-filled) from TCR Cbeta1 negative lymphocytes(black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using Anti-TCR C beta 1 Antibody (FITC) (4 µl reagent / 100 µl of peripheral whole blood).
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