Anti-Tec Antibody (A12533)

Name: Anti-Tec Antibody
Brand: Antibodies.com
SKU: A12533
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Tec for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-Tec Antibody

Description

Rabbit polyclonal antibody to Tec.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Tec (NP_003206.2).

Sequence

MNFNTILEEILIKRSQQKKKTSPLNYKERLFVLTKSMLTYYEGRAEKKYRKGFIDVSKIKCVEIVKNDDGVIPCQNKYPFQVVHDANTLYIFAPSPQSRDLWVKKLKEEIKNNNNIMIKYHPKFWTDGSYQCCRQTEKLAPGCEKYNLFESSIRKALPPAPETKKRRPPPPIPLEEEDNSEEIVVAMYDFQAAEGHDLRLERGQEYLILEKNDVHWWRARDKYGNEGYIPSNYVTGKKSNNLDQYEWYCRNMNRSKAEQLLRSEDKEGGFMVRDSSQPGL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

68 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

MGC126760, MGC126762, PSCTK4, Tec protein tyrosine kinase, TEC_HUMAN, Tyrosine protein kinase, Tyrosine-protein kinase Tec

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Tec Antibody (A12533)

Western blot analysis of extracts of various cell lines, using Anti-Tec Antibody (A12533) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. DeTection: ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Tec Antibody (A12533)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Tec Antibody (A12533) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Tec Antibody (A12533)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Tec Antibody (A12533) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Tec Antibody (A12533)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Tec Antibody (A12533) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.