Anti-Tenascin C Antibody (A308041)

Name: Anti-Tenascin C Antibody
Brand: Antibodies.com
SKU: A308041
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Tenascin C for WB, IHC and ICC/IF.

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$40 to United States

Lead Time

5-8 Business Days

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Anti-Tenascin C Antibody [T2H5]

Mouse monoclonal [T2H5] antibody to Tenascin C for IHC-P, IP and WB.

Specifications

Name

Anti-Tenascin C Antibody

Description

Rabbit polyclonal antibody to Tenascin C.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1990-2201 of human Tenascin C (NP_002151.2).

Sequence

NGDTTSGLYTIYLNGDKAEALEVFCDMTSDGGGWIVFLRRKNGRENFYQNWKAYAAGFGDRREEFWLGLDNLNKITAQGQYELRVDLRDHGETAFAVYDKFSVGDAKTRYKLKVEGYSGTAGDSMAYHNGRSFSTFDKDTDSAITNCALSYKGAFWYRNCHRVNLMGRYGDNNHSQGVNWFHWKGHEHSIQFAEMKLRPSNFRNLEGRRKRA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

240 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

150 225, Cytotactin, Glioma associated extracellular matrix antigen, Glioma-associated-extracellular matrix antigen, GMEM, GP, GP 150 225, GP 150-225, Hexabrachion, HXB, JI, MGC167029, Miotendinous antigen, Myotendinous antigen, Neuronectin, Tenascin, Tenascin-C, Tenascin-C isoform 14/AD1/16, TenascinC, TENA_HUMAN, TN, TN C, TN-C, TNC

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Tenascin C Antibody (A308041)

Western blot analysis of U-138MG, using Anti-Tenascin C Antibody (A308041) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Tenascin C Antibody (A308041)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Tenascin C Antibody (A308041) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Tenascin C Antibody (A308041)

Immunofluorescence analysis of U-87MG cells using Anti-Tenascin C Antibody (A308041) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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