Anti-TFII I Antibody (A15962)

Name: Anti-TFII I Antibody
Brand: Antibodies.com
SKU: A15962
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to TFII I for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TFII I Antibody

Description

Rabbit polyclonal antibody to TFII I.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 600-810 of human GTF2I (NP_001157108.1).

Sequence

TWFGIPRLERIVRGSNKIKFVVKKPELVISYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

132 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BAP 135, BAP-135, BAP135, Bruton tyrosine kinase associated protein 135, Bruton tyrosine kinase-associated protein 135, BTK associated protein, BTK associated protein 135, BTK associated protein 135kD, BTK-associated protein 135, BTKAP 1, BTKAP1, DIWS, FLJ38776, FLJ56355, General transcription factor II i, General transcription factor II-I, General transcription factor IIi, GTF 2I, Gtf2i, GTF2I_HUMAN, GTFII I, GTFII-I, IB 291, IB291, SPIN, SRF Phox 1 interacting protein, SRF Phox1 interacting protein, SRF-Phox1-interacting protein, TFII-I, Transcription factor II I, WBS, WBSCR 6, WBSCR6, Williams Beuren syndrome chromosome region 6, Williams Beuren syndrome chromosome region 6 protein, Williams-Beuren syndrome chromosomal region 6 protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-TFII I Antibody (A15962)

Western blot analysis of extracts of BT-474 cells, using Anti-TFII I Antibody (A15962) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-TFII I Antibody (A15962)

Western blot analysis of extracts of mouse brain, using Anti-TFII I Antibody (A15962) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-TFII I Antibody (A15962)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-TFII I Antibody (A15962) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-TFII I Antibody (A15962)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-TFII I Antibody (A15962) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.