Anti-TFIIB Antibody (A89343)

Name: Anti-TFIIB Antibody
Brand: Antibodies.com
SKU: A89343
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to TFIIB for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-TFIIB Antibody

Description

Rabbit polyclonal antibody to TFIIB.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-154 of GTF2B (NP_001505.1).

Sequence

VVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

General transcription factor IIB, General transcription factor TFIIB, gtf2b, RNA polymerase II transcription factor IIB, S300 II, S300-II, TF IIB, TF2B, TF2B_HUMAN, Transcription initiation factor IIB

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-TFIIB Antibody (A89343)

Western blot analysis of extracts of various cell lines, using Anti-TFIIB Antibody (A89343) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-TFIIB Antibody (A89343)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-TFIIB Antibody (A89343)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-TFIIB Antibody (A89343)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-TFIIB Antibody (A89343) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.