Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567)

Name: Anti-Thrombomodulin Antibody [ARC52599 + ARC52600]
Brand: Antibodies.com
SKU: A309567

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This product is discontinued

Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) has been discontinued and is no longer available.

View all Anti-Thrombomodulin Antibodies.

Specifications

Name

Anti-Thrombomodulin Antibody [ARC52599 + ARC52600]

Description

Rabbit Oligoclonal [ARC52599 + ARC52600] antibody to Thrombomodulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:6,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant protein of human CD141/Thrombomodulin.

Host

Rabbit

Clonality

Oligoclonal

Clone ID

ARC52599 + ARC52600

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AHUS 6, AHUS6, BDCA 3, BDCA3, CD 141, CD141, CD141 antigen, Fetomodulin, Thbd, THPH12, THRM, TM, TRBM_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567)

Western blot analysis of extracts of A-431, using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567)

Immunohistochemistry analysis of paraffin-embedded human lung squamous carcinoma tissue using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Thrombomodulin Antibody [ARC52599 + ARC52600] (A309567) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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