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Anti-Tissue Factor Antibody (A16814)

Western Blot - Anti-Tissue Factor Antibody (A16814) - Antibodies.com Western Blot - Anti-Tissue Factor Antibody (A16814) - Antibodies.com Immunofluorescence - Anti-Tissue Factor Antibody (A16814) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Tissue Factor for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Tissue Factor Antibody
Description
Rabbit polyclonal antibody to Tissue Factor.
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human Coagulation factor III/Tissue Factor (NP_001984.1).
Sequence
SAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
53 kDa / 45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD142, CD142 antigen, Coagulation factor III, Coagulation factor III (thromboplastin tissue factor), F3, FLJ17960, TF, TFA, TF_HUMAN, Thromboplastin
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Tissue Factor Antibody (A16814) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Tissue Factor Antibody (A16814)
Western blot analysis of extracts of BxPC-3 cells, using Anti-Tissue Factor Antibody (A16814) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Tissue Factor Antibody (A16814) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Tissue Factor Antibody (A16814)
Western blot analysis of extracts of various cell lines, using Anti-Tissue Factor Antibody (A16814) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-Tissue Factor Antibody (A16814) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Tissue Factor Antibody (A16814)
Immunofluorescence analysis of A431 cells using Anti-Tissue Factor Antibody (A16814) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
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