Anti-TNF Receptor I Antibody (A305105)

Name: Anti-TNF Receptor I Antibody
Brand: Antibodies.com
SKU: A305105
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to TNF Receptor I for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Endothelial Stem Cell Marker

Specifications

Name

Anti-TNF Receptor I Antibody

Description

Rabbit polyclonal antibody to TNF Receptor I.

Specificity

Other bands present may be the result of oligomerization, self-aggregation and/or cleavage of the TNF-R1 extracellular domain.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Monkey, Canine, Rabbit

Immunogen

Peptide corresponding to AA 20-43 of the mouse TNF-R1 sequence, identical to rat and human over those residues.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD120a, FPF, MGC19588, p55, p55-R, p60, TBP1, TBPI, TNF R, TNF R55, TNF-R1, TNF-RI, TNFAR, TNFR-I, TNFR1, TNFR55, TNFR60, TNFRI, TNFRSF1a, TNR1A_HUMAN, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily, member 1A, Tumor necrosis factor receptor type 1, Tumor necrosis factor receptor type I, Tumor necrosis factor-binding protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-TNF Receptor I Antibody (A305105)

Western blot analysis of mouse liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Anti-TNF Receptor I Antibody (A305105) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~55 kDa.

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Figure 2: Western Blot - Anti-TNF Receptor I Antibody (A305105)

Western blot analysis of human A549 showing detection of ~ 50 kDa TNF-R1 protein using Anti-TNF Receptor I Antibody (A305105) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: MW Ladder, Lane 2: A549. Load: 30 ug. Block: 5% BSA in TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~ 50 kDa. Other Band(s): ~90-100kDa. Other bands can be explained by a few factors, such as oligomerization, self-aggregation, cleavage of the TNFR1 extracellular domain, etc.( Literature references: doi: 10.3389/fcell.2020.615141, 10.1128/MCB.22.8.2536-2543.2002, 10.1073/pnas.0307981100).

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Figure 3: Immunofluorescence - Anti-TNF Receptor I Antibody (A305105)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TNF Receptor I Antibody (A305105), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Golgi apparatus membrane. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-TNF-R1 Antibody. (C) Composite.

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Figure 4: Immunofluorescence - Anti-TNF Receptor I Antibody (A305105)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol at -20°C for 10 minutes, using Anti-TNF Receptor I Antibody (A305105), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at room temperature in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm.