Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA topoisomerase 1, DNA topoisomerase I, NUP98 fusion gene, TOP 1, TOP I, TOP1, TOP1_HUMAN, TOPI, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, TopoisomeraseI, Type I DNA topoisomerase
Figure 1: Western Blot - Anti-Topoisomerase I Antibody (A88019)
Western blot analysis of extracts of various cell lines, using Anti-Topoisomerase I Antibody (A88019) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Figure 2: Immunohistochemistry - Anti-Topoisomerase I Antibody (A88019)
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Topoisomerase I Antibody (A88019) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 3: Western Blot - Anti-Topoisomerase I Antibody (A88019)
Immunoprecipitation analysis of 200µg extracts of Jurkat cells using 3µg of Anti-Topoisomerase I Antibody (A88019). This Western blot was performed on the immunoprecipitate using Anti-Topoisomerase I Antibody (A88019) at a dilution of 1:1000.
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