Principle of Assay
This kit provides a convenient tool for detection of total antioxidant capacity in serum, plasma, animal and plant tissues, cells, bacteria, urine, and other biological fluids. The principle is that in an acidic environment, antioxidants can reduce Fe3+-Tripyridine triazine (Fe3+-TPTZ) to produce blue Fe2+-TPTZ. The total antioxidant capacity of samples is obtained by determining the content of Fe2+-TPTZ at 593 nm. Do not use EDTA as the anticoagulant for serum and plasma samples. The samples also can not contain DTT, Mercaptoethanol, Tween or Triton, or NP-40.
