Principle of Assay
This kit was specially developed for the detection of total carbohydrates in animal and plant tissues, serum, plasma, and other liquid samples. The total carbohydrate is hydrolyzed into reducing sugars. The reducing sugars are heated with DNS reagents under alkaline conditions to reduce DNS and generate amino compounds, which are reddish-brown in alkaline solution and have a characteristic absorption peak at 540 nm. Within a certain concentration range, the reducing sugar content has a linear relationship with the absorbance at 540 nm. Total carbohydrates in the sample can be calculated by reference to the standard curve. This kit cannot achieve 100% degradation of cellulose.
