Anti-TRAF2 Antibody [ARC51292] (A305567)

Name: Anti-TRAF2 Antibody [ARC51292]
Brand: Antibodies.com
SKU: A305567
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51292] antibody to TRAF2 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-TRAF2 Antibody [ARC51292]

Description

Rabbit monoclonal [ARC51292] antibody to TRAF2.

Applications

WB, IHC, IP

Dilutions

WB: 1:10,000-1:60,000, IHC: 1:100-1:500, IP: 1:10,000-1:60,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human TRAF2 (NP_066961.2).

Sequence

DGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTY

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51292

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 ubiquitin-protein ligase TRAF2, MGC:45012, OTTHUMP00000022625, OTTHUMP00000064745, TNF receptor associated factor 2, TNF receptor-associated factor 2, TNF receptor-associated protein, TRAF 2, TRAF2_HUMAN, TRAP, TRAP 3, TRAP3, Tumor necrosis factor type 2 receptor associated protein 3, Tumor necrosis factor type 2 receptor-associated protein 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-TRAF2 Antibody [ARC51292] (A305567)

Western blot analysis of various lysates, using Anti-TRAF2 Antibody [ARC51292] (A305567) at 1:50,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-TRAF2 Antibody [ARC51292] (A305567)

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Figure 3: Western Blot - Anti-TRAF2 Antibody [ARC51292] (A305567)

Western blot analysis of extracts from wild type(WT) and TRAF2 knockdown (KD) 293T cells, using Anti-TRAF2 Antibody [ARC51292] (A305567) at 1:50,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-TRAF2 Antibody [ARC51292] (A305567)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-TRAF2 Antibody [ARC51292] (A305567) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.