Anti-Transferrin Antibody [HTF-14] (A280668)

Anti-Transferrin Antibody [HTF-14]

Mouse monoclonal [HTF-14] antibody to Transferrin.

This antibody recognises the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognises a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division.

ELISA, WB, IF, Functional Assays

Human, Pig, Rabbit, Rat

This antibody does not cross react with Sheep, Bovine, Dog, or Horse

Purified porcine transferrin.

Mouse

Monoclonal

HTF-14

IgG1

Unconjugated

Protein A affinity chromatography of ascites.

Approximately 77 kDa under reducing conditions in human plasma.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti transferrin antibody, clone HTF-14 recognizes the N-terminal domain of human transferrin and has been shown to block binding of transferrin to the transferrin receptor (CD71). Clone HTF-14 is not able to recognize receptor bound transferrin. Clone HTF-14 recognizes a conformational epitope in a loop of the N lobe of transferrin involving the critical Lys-144 residue, also essential for binding of the N lobe to the transferrin receptor (Mason et al.2009)Transferrin is a 77 kDa plasma protein synthesized by the liver involved with the transport of iron. Each transferrin molecule has two domains, both with facility to carry 2 ferric ions. The iron/transferrin complex is essential for hemoglobin synthesis and certain types of cell division.

Apotransferrin, ATRAF, Atransferrinemia, Beta 1 metal binding globulin, Beta-1 metal-binding globulin, DKFZp781D0156, HEL S 71p, HELS71p, PRO1400, PRO1557, PRO2086, Serotransferrin, Serotransferrin precursor, Siderophilin, TF, TFQTL1, Transferin, TRFE_HUMAN