Anti-Transferrin Receptor Antibody [8D3] (FITC) (A279545)

Anti-Transferrin Receptor Antibody [8D3] (FITC)

Rat monoclonal [8D3] antibody to Transferrin Receptor (FITC).

This antibody recognises mouse CD71, a 763 amino acid ~95 kDa single pass type II cell surface transmembrane glycoprotein, otherwise known as the transferrin receptor. CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.recognises native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells.Rat anti Mouse CD71 antibody, clone 8D3 has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse.

Flow Cytometry, IHC-Fr, Functional Assays

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶

Mouse

Mouse transformed endothelioma cell line (tEnd.1).

Rat

Monoclonal

8D3

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD71 antibody, clone 8D3 recognizes mouse CD71, a 763 amino acid ~95 kDa single pass type II cell surface transmembrane glycoprotein, otherwise known as the transferrin receptor. CD71 is a major iron-binding protein, which plays a key role in the transport of iron into cells that require it. In mice, CD71 is widely expressed on a variety of cells, including Sertoli cells and cells which form the blood brain barrier (BBB) in the central nervous system.Rat anti Mouse CD71 antibody, clone 8D3 recognizes native, soluble and denatured forms of murine CD71. Binding of the 8D3 antibody to CD71 does not inhibit the proliferation of cell lines tested, and does not interfere with the uptake of iron into cells.Rat anti Mouse CD71 antibody, clone 8D3 has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse (Kissel et al. 1988).

CD 71, CD71, CD71 antigen, IMD46, OTTHUMP00000208523, OTTHUMP00000208524, OTTHUMP00000208525, p90, sTfR, T9, TfR, TFR 1, TfR1, TFR1_HUMAN, TFRC, TR, Transferrin receptor (p90 CD71), Transferrin receptor protein 1, serum form, Trfr

This product is for research use only. It is not intended for diagnostic or therapeutic use.