Anti-Transferrin Receptor Antibody [ARC54396] (A308474)

Western blot analysis of HeLa, using Anti-Transferrin Receptor Antibody [ARC54396] (A308474) at 1:80000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

Western blot analysis of various lysates, using Anti-Transferrin Receptor Antibody [ARC54396] (A308474) at 1:80000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Transferrin Receptor Antibody [ARC54396] (A308474) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Confocal imaging of HeLa cells using Anti-Transferrin Receptor Antibody [ARC54396] (A308474), at a dilution of 1:200, (red). The cells were counterstained with Anti-CD44 Antibody, at a dilution of 1:200, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.