Anti-TRAP1 Antibody (A305020)

Name: Anti-TRAP1 Antibody
Brand: Antibodies.com
SKU: A305020
Price: 465.0 USD
Availability: InStock

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$465

Rabbit polyclonal antibody to TRAP1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-TRAP1 Antibody

Description

Rabbit polyclonal antibody to TRAP1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:120

Reactivity

Human, Mouse, Rat

Immunogen

Human recombinant TRAP1 (amino acids 1-59 removed - transit peptide).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A purification.

Concentration

1 mg/ml

Molecular Weight

~75 kDa (multiple isoforms)

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Heat shock protein 75 kDa, Heat shock protein 75 kDa, mitochondrial, HSP 75, HSP75, HSP90L, mitochondrial, TNF receptor associated protein 1, TNFR-associated protein 1, TRAP-1, TRAP1_HUMAN, Tumor necrosis factor type 1 receptor-associated protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-TRAP1 Antibody (A305020)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of ~75 kDa TRAP1 protein using Anti-TRAP1 Antibody (A305020) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: human Cervical cancer cell line (HeLa) lysate. Load: 12 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~75 kDa. Other Band(s): 100 kDa.

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Figure 2: Immunofluorescence - Anti-TRAP1 Antibody (A305020)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TRAP1 Antibody (A305020), at 1:120 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 3: Immunofluorescence - Anti-TRAP1 Antibody (A305020)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-TRAP1 Antibody (A305020), at 1:120 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-TRAP1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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