Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724)

Name: Anti-Tropomyosin 1 (alpha) Antibody [ARC1283]
Brand: Antibodies.com
SKU: A306724
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1283] antibody to Tropomyosin 1 (alpha) for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Tropomyosin 1 (alpha) Antibody [ARC1283]

Description

Rabbit monoclonal [ARC1283] antibody to Tropomyosin 1 (alpha).

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 185-284 of human Tropomyosin 1 (P09493).

Sequence

LSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1283

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

37 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AA986836, AI854628, Alpha tropomyosin, alpha-TM, Alpha-tropomyosin, C15orf13, cardiomyopathy, hypertrophic 3, CMD1Y, CMH3, HTM alpha, HTM-alpha, OTTHUMP00000163688, sarcomeric tropomyosin kappa, TM2, Tmpa, TMSA, Tpm-1, TPM1, TPM1_HUMAN, Tropomyosin 1, tropomyosin 1 (alpha) isoform 1, tropomyosin 1 (alpha) isoform 2, tropomyosin 1 (alpha) isoform 3, tropomyosin 1 (alpha) isoform 4, tropomyosin 1 (alpha) isoform 5, tropomyosin 1 (alpha) isoform 6, tropomyosin 1 (alpha) isoform 7, Tropomyosin alpha 1 chain, Tropomyosin alpha-1 chain, Tropomyosin, skeletal muscle alpha, Tropomyosin-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724)

Western blot analysis of extracts of various cell lines, using Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724)

Immunofluorescence analysis of rat heart using Anti-Tropomyosin 1 (alpha) Antibody [ARC1283] (A306724) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).