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Anti-TRP 7 Antibody [N64A/36] (A304950)

Immunocytochemistry/Immunofluorescence - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.com Western Blot - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.com Immunohistochemistry - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.com
$500
Product Datasheet

Mouse monoclonal [N64A/36] antibody to TRP 7 for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-TRP 7 Antibody [N64A/36]
Description
Mouse monoclonal [N64A/36] antibody to TRP 7.
Applications
WB, IHC, ICC/IF, IP, Antibody Microarray
Dilutions
WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide amino acids 845-862 of human TRPC7.
Host
Mouse
Clonality
Monoclonal
Clone ID
N64A/36
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~100 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This antibody was formely sold under the Clone ID S64A-36.
Synonyms
Capacitative calcium channel TRPC7, cation channel, subfamily C, member 7, hTRP7, KNP3, Likley ortholog of mouse transient receptor potential cation channel subfamily C member 7, mTRP7, Putative capacitative calcium channel, Short transient receptor potential channel 7, Transient receptor potential cation channel subfamily C member 7, transient receptor potential-related channel 7, a novel putative Ca2+ channel protein, Transient receptor protein 7, TRP-7, TRP7 protein, TRPC 7, TrpC7, Trpc7 transient receptor potential cation channel, subfamily C, member 7, TRPC7_HUMAN, TRPM2, Trrp8
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunocytochemistry/Immunofluorescence - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.comEnlarge Image
Figure 1: Immunofluorescence - Anti-TRP 7 Antibody [N64A/36] (A304950)
Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-TRP 7 Antibody [N64A/36] (A304950), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Nuclear staining.
Western Blot - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-TRP 7 Antibody [N64A/36] (A304950)
Western blot analysis of rat brain membrane lysate showing detection of TrpC7 protein using Anti-TRP 7 Antibody [N64A/36] (A304950) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.
Immunohistochemistry - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-TRP 7 Antibody [N64A/36] (A304950)
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-TRP 7 Antibody [N64A/36] (A304950) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Everything.
Immunocytochemistry/Immunofluorescence - Anti-TRP 7 Antibody [N64A/36] (A304950) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-TRP 7 Antibody [N64A/36] (A304950)
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-TRP 7 Antibody [N64A/36] (A304950), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 Antibody (D) Composite.
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