Anti-Tubulin Antibody (A80592)

Name: Anti-Tubulin Antibody
Brand: Antibodies.com
SKU: A80592
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Tubulin for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Microtubule Marker

Specifications

Name

Anti-Tubulin Antibody

Description

Rabbit polyclonal antibody to Tubulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence within amino acids 1-80 of human Alpha-tubulin (ubiquitous) chain (NP_006073.2).

Sequence

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha tubulin, Alpha-tubulin ubiquitous, beta Ib tubulin, CDCBM5, CDCBM6, fd02b12, K ALPHA 1, M40, OK/SW-cl.56, TBA1B_HUMAN, TUBA1B, TUBB1, TUBB2, TUBB5, tubulin alpha 1b, Tubulin alpha-1 chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-ubiquitous chain, Tubulin beta, Tubulin beta 1b, Tubulin beta 2A, tubulin beta 2A class IIa, Tubulin beta 2b, tubulin beta chain, tubulin beta class I, tubulin beta-1 chain, tubulin beta-2A chain, tubulin beta-5 chain, Tubulin K-alpha-1, tubulin, alpha, ubiquitous, tubulin, beta 2A class IIa, tubulin, beta polypeptide, tubulin, beta polypeptide 2, tubulin, beta, class IIA, wu:fd02b12, zgc:55461

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Tubulin Antibody (A80592)

Western blot analysis of extracts of various cell lines, using Anti-Tubulin Antibody (A80592) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-Tubulin Antibody (A80592)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Tubulin Antibody (A80592)Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain antibody (AC024) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Tubulin Antibody (A80592)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Tubulin Antibody (A80592)Immunohistochemistry analysis of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain antibody (AC024) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Tubulin Antibody (A80592)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Tubulin Antibody (A80592) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).