Principle of Assay
This Turkey IGF-1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Turkey IGF-1.
An antibody specific for IGF-1 has been coated on a microplate. Standards and samples are pipetted into the wells and the IGF-1 present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-IGF-1 Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of IGF-1 bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
