Anti-Twist Antibody (A88733)

Name: Anti-Twist Antibody
Brand: Antibodies.com
SKU: A88733
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Twist for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Twist Antibody

Description

Rabbit polyclonal antibody to Twist.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of Twist (NP_000465.1).

Sequence

MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACS3, B-HLH DNA binding protein, bHLHa38, BPES2, BPES3, Class A basic helix-loop-helix protein 38, CRS, CRS1, CSO, H-twist, OTTHUMP00000116043, SCS, Twist basic helix loop helix transcription factor 1, Twist family bHLH transcription factor 1, Twist homolog 1, Twist homolog 1 (Drosophila), TWIST homolog of drosophila, Twist related protein 1, Twist-related protein 1, TWIST1, TWST1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Twist Antibody (A88733)

Western blot analysis of various lysates, using Anti-Twist Antibody (A88733) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-Twist Antibody (A88733)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Twist Antibody (A88733) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Twist Antibody (A88733)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Twist Antibody (A88733) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Twist Antibody (A88733)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-Twist Antibody (A88733) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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