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Anti-UBA3 Antibody [ARC2865] (A306848)

Western Blot - Anti-UBA3 Antibody [ARC2865] (A306848) - Antibodies.com Western Blot - Anti-UBA3 Antibody [ARC2865] (A306848) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2865] antibody to UBA3 for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-UBA3 Antibody [ARC2865]
Description
Rabbit monoclonal [ARC2865] antibody to UBA3.
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human UBA3 (Q8TBC4).
Sequence
MADGEEPEKKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTCKVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2865
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
52 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DKFZp566J164, EC 6.3.2., hUba3, MGC22384, NEDD8 activating enzyme E1 catalytic subunit, NEDD8 activating enzyme E1C, Nedd8 activating enzyme hUba3, NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, UBA3 ubiquitin activating enzyme E1 homolog, UBA3_HUMAN, UBE1C, Ubiquitin activating enzyme 3, Ubiquitin activating enzyme E1C, Ubiquitin-activating enzyme 3, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-UBA3 Antibody [ARC2865] (A306848) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-UBA3 Antibody [ARC2865] (A306848)
Western blot analysis of extracts of various cell lines, using Anti-UBA3 Antibody [ARC2865] (A306848) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-UBA3 Antibody [ARC2865] (A306848) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-UBA3 Antibody [ARC2865] (A306848)
Western blot analysis of extracts of Rat heart, using Anti-UBA3 Antibody [ARC2865] (A306848) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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