Anti-UBE2B Antibody [PCRP-UBE2B-1C7] (A250264)

Mouse monoclonal [PCRP-UBE2B-1C7] antibody to UBE2B for Flow Cytometry and IF.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-UBE2B Antibody [PCRP-UBE2B-1C7]
Description
Mouse monoclonal [PCRP-UBE2B-1C7] antibody to UBE2B.
Specificity
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2A (ubiquitin-conjugating enzyme E2 A) and UBE2B (ubiquitin-conjugating enzyme E2 B) are both Ub-conjugating enzymes that are essential to post replication repair of UV-damaged DNA. UBE2A and UBE2B are both nuclear and cell membrane proteins that have been found to interact with Rad18.
Applications
Flow Cytometry, IF
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml
Reactivity
Human
Cross Reactivity
This antibody is predicted to cross react with Mouse, Rat, Xenopus, and Zebrafish.
Immunogen
Recombinant full-length human UBE2B protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
PCRP-UBE2B-1C7
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
17 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-UBE2B Antibody [PCRP-UBE2B-1C7] - BSA and Azide free (A253444).
Synonyms
E2 17kDa, E2 protein, hHR6B, HR6B, RAD6 homolog B, RAD6B, UBC2, UBE2B_HUMAN, Ubiquitin carrier protein B, Ubiquitin conjugating enzyme E2 B, Ubiquitin conjugating enzyme E2B, Ubiquitin conjugating enzyme E2B (RAD6 homolog), Ubiquitin protein ligase B, Ubiquitin-conjugating enzyme E2 17 kDa, Ubiquitin-conjugating enzyme E2 B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase B
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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