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Anti-UBE2O Antibody (A11220)

Western Blot - Anti-UBE2O Antibody (A11220) - Antibodies.com Western Blot - Anti-UBE2O Antibody (A11220) - Antibodies.com Immunofluorescence - Anti-UBE2O Antibody (A11220) - Antibodies.com Western Blot - Anti-UBE2O Antibody (A11220) - Antibodies.com
Knockout Validated
$470
Product Datasheet

Rabbit polyclonal antibody to UBE2O for WB, ICC/IF and IP.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-UBE2O Antibody
Description
Rabbit polyclonal antibody to UBE2O.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:1,000-1:4,000, ICC/IF: 1:50-1:200, IP: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
Sequence
LVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
200 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
E2 230K, EC=6.3.2.19, FLJ12878, KIAA1734, Likely Ortholog of Mouse Ubiquitin Conjugating Enzyme, RP23 193A16.5, UBE2O_HUMAN, Ubiquitin carrier protein O, Ubiquitin conjugating enzyme E2 O, Ubiquitin conjugating enzyme E2 of 230 kDa, ubiquitin conjugating enzyme E2O, Ubiquitin protein ligase O, Ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 of 230 kDa, Ubiquitin-conjugating enzyme E2-230K, Ubiquitin-protein ligase O
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-UBE2O Antibody (A11220) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-UBE2O Antibody (A11220)
Western blot analysis of extracts of various cell lines, using Anti-UBE2O Antibody (A11220) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-UBE2O Antibody (A11220) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-UBE2O Antibody (A11220)
Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using Anti-UBE2O Antibody (A11220) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-UBE2O Antibody (A11220) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-UBE2O Antibody (A11220)
Immunofluorescence analysis of HeLa cells using Anti-UBE2O Antibody (A11220) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-UBE2O Antibody (A11220) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Anti-UBE2O Antibody (A11220)
Immunoprecipitation analysis of 200µg extracts of A-431 cells using 3µg of Anti-UBE2O Antibody (A11220). This Western blot was performed on the immunoprecipitate using Anti-UBE2O Antibody (A11220) at a dilution of 1:1000.
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