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Anti-UHRF1 Antibody

Rabbit polyclonal antibody to UHRF1 from ABclonal (A0297).
Name: Anti-UHRF1 Antibody
Description: Rabbit polyclonal antibody to UHRF1.
Applications: WB, IF, FC
Dilutions: WB: 1:500 - 1:2000, IF: 1:20 - 1:50, FC: 1:20 - 1:50.
Reactivity: Human
Immunogen: A synthetic peptide of human UHRF1.
Protein Length: 793
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Affinity purification.
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Function: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
Tissue Specificity: Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
Post-Translational Modification: Phosphorylation at Ser-298 of the linker region decreases the binding to H3K9me3. Phosphorylation at Ser-639 by CDK1 during M phase impairs interaction with USP7, preventing deubiquitination and leading to degradation by the proteasome.
Cellular Location: Nucleus.

Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions.
UniProt: Q96T88
Gene ID: 29128
Synonyms: Ac2-121 Antibody
AL022808 Antibody
E3 ubiquitin-protein ligase UHRF1 Antibody
EC 6.3.2.- Antibody
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hNP95 Antibody
hUHRF1 Antibody
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MGC138707 Antibody
NP95 Antibody
Nuclear phosphoprotein, 95-KD Antibody
Nuclear protein 95 Antibody
Nuclear zinc finger protein Np95 Antibody
RING finger protein 106 Antibody
RNF106 Antibody
Transcription factor ICBP90 Antibody
Ubiquitin like containing PHD and RING finger domains protein 1 Antibody
Ubiquitin like PHD and RING finger domain containing protein 1 Antibody
Ubiquitin-like PHD and RING finger domain-containing protein 1 Antibody
Ubiquitin-like protein containing PHD and RING finger domains 1 Antibody
Ubiquitin-like with PHD and ring finger domains 1 Antibody
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UHRF1_HUMAN Antibody
Information: Target information shown above is from the UniProt Consortium.
Western blot analysis of extracts of MCF7 cell line, using UHRF1 antibody.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.
Description: Rabbit polyclonal antibody to UHRF1.
Application: WB, IHC, IF, IP, ChIP
Host: Rabbit
Isotype: IgG
Reactivity: Human

£148 – £372

Description: Rabbit polyclonal antibody to UHRF1 (ChIP Grade)
Application: WB, IHC, IF, IP, CHIP
Host: Rabbit
Reactivity: Human
Immunogen: Recombinant full length Human UHRF1.

£220 – £366

Description: Mouse monoclonal antibody to UHRF1 (N-term)
Application: WB, ICC
Host: Mouse
Isotype: IgG2b
Reactivity: Human

£185 – £245

Description: Mouse monoclonal antibody to UHRF1 (N-terminus)
Application: WB, ICC
Host: Mouse
Reactivity: Human, Mouse
Immunogen: Synthetic peptide, corresponding to the N-terminus of Human UHRF1.

£220 – £366

Please Note: Anti-UHRF1 Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A0297
Size Price Quantity
100µl £243
200µl £372

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Lead Time: Currently 4-6 Days
Shipping Information
  • £20 to United Kingdom
  • £35 to Europe