Principle of Assay
This kit provides a simple method for determining the uric acid concentration in a variety of biological samples such as animal tissues, serum, plasma, urine, and other biological fluids. In the assay, uricase catalyzes the conversion of uric acid to produce allantoin, carbon dioxide and hydrogen peroxide. The hydrogen peroxide oxidizes Fe2+ in potassium ferrocyanide to produce Fe3+. Fe3+ further reacts with phenol and 4-aminoantipyrine to form red quinone compounds which have a characteristic absorption peak at 505 nm. The uric acid content can be calculated by measuring the light absorption at 505 nm.
