Anti-USP25 Antibody [ARC60125] (A309509)

Name: Anti-USP25 Antibody [ARC60125]
Brand: Antibodies.com
SKU: A309509
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC60125] antibody to USP25 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-USP25 Antibody [ARC60125]

Description

Rabbit monoclonal [ARC60125] antibody to USP25.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:6,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human USP25.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC60125

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Deubiquitinating enzyme 25, Ubiquitin carboxyl-terminal hydrolase 25, Ubiquitin specific peptidase 25, ubiquitin specific protease 25, ubiquitin specific protease USP25, Ubiquitin thiolesterase 25, Ubiquitin-specific-processing protease 25, UBP25_HUMAN, USP on chromosome 21, USP21

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-USP25 Antibody [ARC60125] (A309509)

Western blot analysis of various lysates, using Anti-USP25 Antibody [ARC60125] (A309509) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-USP25 Antibody [ARC60125] (A309509)

Western blot analysis of various lysates, using Anti-USP25 Antibody [ARC60125] (A309509) at 1:6,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Immunohistochemistry - Anti-USP25 Antibody [ARC60125] (A309509)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-USP25 Antibody [ARC60125] (A309509) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-USP25 Antibody [ARC60125] (A309509)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-USP25 Antibody [ARC60125] (A309509) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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