Anti-VAMP1 Antibody [ARC2031] (A307716)

Name: Anti-VAMP1 Antibody [ARC2031]
Brand: Antibodies.com
SKU: A307716

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This product is discontinued

Anti-VAMP1 Antibody [ARC2031] (A307716) has been discontinued and is no longer available.

View all Anti-VAMP1 Antibodies.

Specifications

Name

Anti-VAMP1 Antibody [ARC2031]

Description

Rabbit monoclonal [ARC2031] antibody to VAMP1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human VAMP1 (P23763).

Sequence

MSAPAQPPAEGTEGTAPGGGPPGPPPNMTSNRRLQQTQAQVEEVVDIIRVNVDKVLERDQKLSELDDRADALQAGASQFESSAAKLKRKYWWKNCKMMIMLGAICAIIVVVIVIYFFT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2031

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp686H12131, SYB 1, SYB1, Synaptobrevin 1, Synaptobrevin-1, Synaptobrevin1, VAMP 1, VAMP-1, VAMP1_HUMAN, Vesicle associated membrane protein 1, Vesicle associated membrane protein 1 synaptobrevin 1, Vesicle-associated membrane protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-VAMP1 Antibody [ARC2031] (A307716)

Western blot analysis of extracts of various cell lines, using Anti-VAMP1 Antibody [ARC2031] (A307716) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-VAMP1 Antibody [ARC2031] (A307716)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-VAMP1 Antibody [ARC2031] (A307716) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-VAMP1 Antibody [ARC2031] (A307716)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-VAMP1 Antibody [ARC2031] (A307716) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-VAMP1 Antibody [ARC2031] (A307716)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-VAMP1 Antibody [ARC2031] (A307716) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.