Anti-VCP Antibody [ARC0728] (A307008)

Name: Anti-VCP Antibody [ARC0728]
Brand: Antibodies.com
SKU: A307008
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0728] antibody to VCP for WB and ICC/IF.

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$40 to United States

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5-8 Business Days

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Specifications

Name

Anti-VCP Antibody [ARC0728]

Description

Rabbit monoclonal [ARC0728] antibody to VCP.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VCP (P55072).

Sequence

MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0728

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

97 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

15S Mg(2+) ATPase p97 subunit, 15S Mg(2+)-ATPase p97 subunit, ALS14, ATPase p97, CDC48, IBMPFD, MGC131997, MGC148092, MGC8560, p97, TER ATPase, TERA, TERA_HUMAN, Transitional endoplasmic reticulum ATPase, Valosin containing protein, Valosin-containing protein, Yeast Cdc48p homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-VCP Antibody [ARC0728] (A307008)

Western blot analysis of extracts of various cell lines, using Anti-VCP Antibody [ARC0728] (A307008) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-VCP Antibody [ARC0728] (A307008)

Immunofluorescence analysis of C6 cells using Anti-VCP Antibody [ARC0728] (A307008) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-VCP Antibody [ARC0728] (A307008)

Immunofluorescence analysis of NIH-3T3 cells using Anti-VCP Antibody [ARC0728] (A307008) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-VCP Antibody [ARC0728] (A307008)

Immunofluorescence analysis of U-2 OS cells using Anti-VCP Antibody [ARC0728] (A307008) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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