VEGFR2 (phospho Tyr1214) Cell Based ELISA Kit (A102048)

VEGFR2 (phospho Tyr1214) Cell Based ELISA Kit

Cell-based (qualitative)

This VEGFR2 (phospho Tyr1214) Cell Based ELISA Kit allows for the detection of VEGFR2 (phospho Tyr1214) and the effects that certain stimulation conditions have on VEGFR2 (phospho Tyr1214) expression in different cell lines. Qualitative determination of VEGFR2 (phospho Tyr1214) concentration is achieved by an indirect ELISA format. In essence, the VEGFR2 (phospho Tyr1214) is captured by Anti-VEGFR2 (phospho Tyr1214) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this VEGFR2 (phospho Tyr1214) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-VEGFR2 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

CD309, CD309 antigen, EC 2.7.10.1, Fetal liver kinase 1, FLK-1, FLK1, FLK1, mouse, homolog of, Kdr, Kinase insert domain receptor, Kinase insert domain receptor (a type III receptor tyrosine kinase), KRD1, Ly73, Protein tyrosine kinase receptor FLK1, Protein-tyrosine kinase receptor flk-1, soluble VEGFR2, Tyrosine kinase growth factor receptor, Vascular endothelial growth factor receptor 2, VEGF Receptor 2, VEGFR, VEGFR 2, VEGFR-2, VGFR2_HUMAN