Anti-VGluT1 Antibody [S28-9] (A304823)

Name: Anti-VGluT1 Antibody [S28-9]
Brand: Antibodies.com
SKU: A304823
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S28-9] antibody to VGluT1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-VGluT1 Antibody [S28-9]

Description

Mouse monoclonal [S28-9] antibody to VGluT1.

Applications

WB, IHC

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with VGlut2.

Immunogen

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S28-9

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BNPI, Brain specific Na (+) dependent inorganic phosphate cotransporter, Brain specific Na dependent inorganic phosphate cotransporter, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Slc17a7, Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7, Solute carrier family 17 member 7, Vesicular glutamate transporter 1, VGLU1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-VGluT1 Antibody [S28-9] (A304823)

Immunocytochemistry/Immunofluorescence analysis of human differentiated SH-SY5Y, using Anti-VGluT1 Antibody [S28-9] (A304823), at 1:100. The secondary antibody used was AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue).(A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.

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Figure 2: Western Blot - Anti-VGluT1 Antibody [S28-9] (A304823)

Western blot analysis of rat brain membrane lysate showing detection of VGLUT1 protein using Anti-VGluT1 Antibody [S28-9] (A304823) at 1:1,000.

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Figure 3: Immunohistochemistry - Anti-VGluT1 Antibody [S28-9] (A304823)

Immunohistochemistry analysis of mouse spinal cord, fixed in 4% PFA. The Primary Antibody used was Anti-VGluT1 Antibody [S28-9] (A304823) at 1:500 for 16 hours at room temperature. The secondary antibody used was Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at room temperature. Counterstain: NeuN neuronal stain (green). Magnification: 20X.

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Figure 4: Immunofluorescence - Anti-VGluT1 Antibody [S28-9] (A304823)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-VGluT1 Antibody [S28-9] (A304823), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.

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