Anti-VILIP1 Antibody [ARC54745] (A306054)

Name: Anti-VILIP1 Antibody [ARC54745]
Brand: Antibodies.com
SKU: A306054

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This product is discontinued

Anti-VILIP1 Antibody [ARC54745] (A306054) has been discontinued and is no longer available.

View all Anti-VILIP1 Antibodies.

Specifications

Name

Anti-VILIP1 Antibody [ARC54745]

Description

Rabbit monoclonal [ARC54745] antibody to VILIP1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 2-191 of human VSNL1/VILIP-1 (NP_003376.2).

Sequence

GKQNSKLAPEVMEDLVKSTEFNEHELKQWYKGFLKDCPSGRLNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRVEMLEIIEAIYKMVGTVIMMKMNEDGLTPEQRVDKIFSKMDKNKDDQITLDEFKEAAKSDPSIVLLLQCDIQK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC54745

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

18 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

21 kDa CABP, Hippocalcin like protein 3, Hippocalcin-like protein 3, HLP 3, HLP3, HPCAL 3, HPCAL3, HUVISL1, Neural visinin-like protein 1, Neural visinin-like type 1 protein, Neurocalcin alpha, NVL 1, NVL1, Nvp1, OZ1, Ratnvp1, VILIP, VILIP-1, Visinin, Visinin like 1, Visinin like protein 1, Visinin-like protein 1, VISL 1, VISL1, VISL1_HUMAN, VLP-1, Vnsl1, Vsnl1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-VILIP1 Antibody [ARC54745] (A306054)

Western blot analysis of extracts of various cell lines, using Anti-VILIP1 Antibody [ARC54745] (A306054) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-VILIP1 Antibody [ARC54745] (A306054)

Confocal imaging of U-2 OS cells using Anti-VILIP1 Antibody [ARC54745] (A306054), at a dilution of 1:200, (red). The cells were counterstained with Anti-alpha Tubulin Antibody, at a dilution of 1:400, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.

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