Anti-Villin Antibody [ARC54727] (A307924)

Name: Anti-Villin Antibody [ARC54727]
Brand: Antibodies.com
SKU: A307924

Enlarge Image

This product is discontinued

Anti-Villin Antibody [ARC54727] (A307924) has been discontinued and is no longer available.

View all Anti-Villin Antibodies.

Specifications

Name

Anti-Villin Antibody [ARC54727]

Description

Rabbit monoclonal [ARC54727] antibody to Villin.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-827 of human Villin1 (NP_009058.2).

Sequence

MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDSSLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLLHVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGVVDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWTASNRTSGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPVDSKWLGHFYGGDCYLLLYTYLIGEKQHYLLYVWQGSQASQDEITASAYQAVILDQKYNGEPVQIRVPMGKEPPHLMSIFKGRMVVYQGGTSRTNNLETGPSTRLFQVQGTGANNTKAFEVPARANFLNSNDVFVLKTQSCCYLWCGKGCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENLVITPRLFECSNKTGRFLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVKQGHEPPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQITAEVTSPKVDVFNANSNLSSGPLPIFPLEQLVNKPVEELPEGVDPSRKEEHLSIEDFTQAFGMTPAAFSALPRWKQQNLKKEKGLF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC54727

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

93 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

D2S1471, OTTHUMP00000164145, VIL, VIL1, VILI_HUMAN, Villin 1, Villin-1, Villin1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-Villin Antibody [ARC54727] (A307924)

Western blot analysis of extracts of various cell lines, using Anti-Villin Antibody [ARC54727] (A307924) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Figure 2: Western Blot - Anti-Villin Antibody [ARC54727] (A307924)

Western blot analysis of extracts of Rat pancreas, using Anti-Villin Antibody [ARC54727] (A307924) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Villin Antibody [ARC54727] (A307924)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Villin Antibody [ARC54727] (A307924) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Villin Antibody [ARC54727] (A307924)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Villin Antibody [ARC54727] (A307924) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.