Anti-Visfatin Antibody [ARC53731] (A309128)

Name: Anti-Visfatin Antibody [ARC53731]
Brand: Antibodies.com
SKU: A309128
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC53731] antibody to Visfatin for WB and IHC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-Visfatin Antibody [ARC53731]

Description

Rabbit monoclonal [ARC53731] antibody to Visfatin.

Applications

WB, IHC

Dilutions

WB: 1:10,000-1:130,000, IHC: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human PBEF/Visfatin/NAMPT (NP_005737.1).

Sequence

VSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53731

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1110035O14Rik, AI314458, AI480535, DKFZP666B131, EC 2.4.2.12, MGC117256, NAmPRTase, Nampt, NAMPT_HUMAN, Nicotinamide phosphoribosyltransferase, PBEF, PBEF 1, PBEF1, Pre B cell colony enhancing factor, Pre B cell colony enhancing factor 1, Pre B cell enhancing factor, Pre-B cell-enhancing factor, Pre-B-cell colony-enhancing factor 1, VF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-Visfatin Antibody [ARC53731] (A309128)

Western blot analysis of extracts of U-87MG cells, using Anti-Visfatin Antibody [ARC53731] (A309128) at 1:110000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

Enlarge Image

Figure 2: Western Blot - Anti-Visfatin Antibody [ARC53731] (A309128)

Western blot analysis of extracts of various cell lines, using Anti-Visfatin Antibody [ARC53731] (A309128) at 1:110000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Visfatin Antibody [ARC53731] (A309128)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Visfatin Antibody [ARC53731] (A309128) at a dilution of 1:1000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Visfatin Antibody [ARC53731] (A309128)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Visfatin Antibody [ARC53731] (A309128) at a dilution of 1:1000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.