Anti-Vitamin D Receptor Antibody [ARC60266] (A307189)

Name: Anti-Vitamin D Receptor Antibody [ARC60266]
Brand: Antibodies.com
SKU: A307189

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This product is discontinued

Anti-Vitamin D Receptor Antibody [ARC60266] (A307189) has been discontinued and is no longer available.

View all Anti-Vitamin D Receptor Antibodies.

Specifications

Name

Anti-Vitamin D Receptor Antibody [ARC60266]

Description

Rabbit monoclonal [ARC60266] antibody to Vitamin D Receptor.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human VDR.

Sequence

MEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC60266

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa / 54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

1, 1 25 dihydroxyvitamin D3 receptor, 1,25 dihydroxyvitamin D3 receptor, 1,25-@dihydroxyvitamin D3 receptor, 25-dihydroxyvitamin D3 receptor, Member 1, NR1I1, Nuclear receptor subfamily 1 group I member 1, PPP1R163, Protein phosphatase 1, regulatory subunit 163, VDR, VDR_HUMAN, Vitamin D (1,25- dihydroxyvitamin D3) receptor, Vitamin D hormone receptor, Vitamin D nuclear receptor variant 1, Vitamin D3 receptor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Vitamin D Receptor Antibody [ARC60266] (A307189)

Western blot analysis of various lysates, using Anti-Vitamin D Receptor Antibody [ARC60266] (A307189) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-Vitamin D Receptor Antibody [ARC60266] (A307189)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Vitamin D Receptor Antibody [ARC60266] (A307189) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Vitamin D Receptor Antibody [ARC60266] (A307189)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Vitamin D Receptor Antibody [ARC60266] (A307189) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Western Blot - Anti-Vitamin D Receptor Antibody [ARC60266] (A307189)

Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-Vitamin D Receptor Antibody [ARC60266] (A307189). This Western blot was performed on the immunoprecipitate using Anti-Vitamin D Receptor Antibody [ARC60266] (A307189) at a dilition of 1:1000.

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