Anti-VPS28 Antibody [ARC2399] (A306398)

Name: Anti-VPS28 Antibody [ARC2399]
Brand: Antibodies.com
SKU: A306398
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2399] antibody to VPS28 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-VPS28 Antibody [ARC2399]

Description

Rabbit monoclonal [ARC2399] antibody to VPS28.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 122-221 of human VPS28 (Q9UK41).

Sequence

KGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2399

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting 28, Vacuolar protein sorting 28 (yeast), Vacuolar protein sorting 28 homolog, Vacuolar protein sorting 28 homolog (S. cerevisiae), Vacuolar protein sorting-associated protein 28 homolog, VPS 28, VPS28_HUMAN, Yeast class E protein Vps28p homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-VPS28 Antibody [ARC2399] (A306398)

Western blot analysis of extracts of various cell lines, using Anti-VPS28 Antibody [ARC2399] (A306398) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-VPS28 Antibody [ARC2399] (A306398)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-VPS28 Antibody [ARC2399] (A306398) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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