Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
C13orf9, CGI 145, EAP45, ELL associated protein of 45 kDa, ELL-associated protein of 45 kDa, ESCRT-II complex subunit VPS36, Vacuolar protein sorting associated protein 36, Vacuolar protein-sorting-associated protein 36, VPS36_HUMAN
Figure 1: Western Blot - Anti-VPS36 Antibody (A92484)
Western blot analysis of extracts of various cell lines, using Anti-VPS36 Antibody (A92484) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of NIH-3T3 cells using Anti-VPS36 Antibody (A92484) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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