Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)

Name: Anti-Wilms Tumor Protein Antibody [ARC2610]
Brand: Antibodies.com
SKU: A308215
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC2610] antibody to Wilms Tumor Protein for WB and IHC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-Wilms Tumor Protein Antibody [ARC2610]

Description

Rabbit monoclonal [ARC2610] antibody to Wilms Tumor Protein.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544).

Sequence

PPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2610

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

51 - 60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AWT1, FWT1, GUD, NPHS4, WAGR, Wilms Tumor, Wilms tumor 1, Wilms' tumor gene, Wilms' tumor protein, WIT 2, WIT2, WT, WT 1, WT1, WT1_HUMAN, WT33

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

Enlarge Image

Figure 1: Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)

Western blot analysis of extracts of various cell lines, using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Figure 2: Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)

Western blot analysis of extracts of Rat testis, using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 3: Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)

Western blot analysis of extracts of Mouse testis, using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.